Obesity affects the auditory system, altering its functionality and auditory processing. Inflammation and the NLRP3 inflammasome may play a role in the development of sensorineural hearing loss (HL) induced by obesity. We aimed to evaluate the effect of obesity induced by high-fat diet (HFD) consumption on the functionality of the auditory system, hair cell survival and inflammation. For that, 7 weeks old male C57BL/6J mice (n=20) were fed a control diet (CD, 10% fat, 20% protein, and 70% carbohydrates) or a HFD (60% fat, 20% protein, and 20% carbohydrates), for 16 weeks. Weight, adipose tissue (AT) and liver histology, distortion product otoacoustic emissions (DPOAEs), auditory brainstem response (ABR), cochlear hair cells numbers and integrity (IF), proinflammation cytokines (interleukin (IL)-6 and TNF-α (qPCR), and NLRP3 inflammasome (NLRP3, ASC, Caspase-1, IL-1β and IL-18, IF and qPCR) were measured. Animals fed a HFD (p<0.05) increased body weight (84%), developed hepatic steatosis, AT dysfunction, compared to CD. Moreover, HFD increased the 2F1-F2 DPOAE threshold by 12,3 dB (p<0.05) and decreased cochlear outer cells number. Cochlear mRNA levels of IL-1β, IL18 and IL-6 were also increased. This data suggests a role for inflammation and NLRP3 inflammasome in hearing impairment induced by metabolic dysfunction, after the chronic consumption of a high-fat diet in C57BL/6J mice.