Neural stem cells (NSCs) have the capacity to differentiate into various neural cell types, including neurons, astrocytes, and oligodendrocytes. This property positions them as a fundamental component in strategies aimed at restoring or enhancing nervous system function following injury or disease. Understanding the molecular mechanisms that regulate NSC differentiation is essential for advancing approaches in neural repair and regeneration. Previous studies have indicated that phosphatidylcholine can induce neuronal differentiation, potentially through the activation of the PKA/CREB signaling pathway, a key regulator of gene expression during neuronal development. In this context, and through the use of molecular and cellular biology tools, this study proposes a molecular mechanism by which phosphatidylcholine promotes neuronal differentiation in NSCs. Elucidating this mechanism may provide new insights into the physiology of neural stem cells and into molecular pathways capable of modulating their behavior.