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Neurochemistry and Neuropharmacology

Posters List

V-103
Neuroprotective effect of Cannabidiol (CBD), modulated by autophagic pathways, in a progressive model of Parkinsonism
José Leandro Santos Souza1, Ana Cleia Alves de Luz1, Heitor Franco Santos1, Cássia Ellen de Jesus Lima1, Adson de Brito Pereira1, José Carlos Junio da Silva Lima1, Tamiris Rodrigues Santos1, Abraão de Jesus Barbosa1, Mylaine Santos Mendonça1, Katty Anne Amador de Lucena Medeiros1, Regina Helena da Silva2, Gustavo José da Silva Pereira2, Ingrid Kazue Mizuno Watanabe2, Vanessa Costhek Abilio2, Alessandra Mussi Ribeiro2, Auderlan Mendonça de Góis1, José Ronaldo dos Santos1
  1. UFS - Universidade Federal de Sergipe
  2. UNIFESP - Universidade Federal de São Paulo
Presenting Author:
José Leandro Santos Souza
leandrosouza.ufs@gmail.com
Parkinson's disease (DP) is a neurodegenerative condition with a decline in processes essential for cellular homeostasis, such as autophagy. Cannabidiol (CBD) has been associated with autophagic activity. Therefore, the focus of this study was to observe the action of CBD in a progressive model of PD in rats. 72 Wistar rats were used and randomly divided into four groups: Control (CTL); Cannabidiol 5mg/kg (CBD); Reserpine 0.5 mg/kg (RES) and Reserpine 0.5 mg/kg + Cannabidiol 5mg/kg (RES+CBD). The animals received 15 subcutaneous administrations of RES or vehicle every 48 hours, and 30 intraperitoneal administrations of CBD or vehicle daily. Behavioral tests were carried out throughout the experiment Immunohistochemistry for tyrosine hydroxylase (TH), GFAP and western blot for α-synuclein (α-syn), TH, LC3-I, LC3-II and p62. The procedures were previously approved by the UFS Animal Research Ethics Committee (CEUA/UFS), under protocol no. CBD was able to minimize the deleterious effects of RES on motor symptoms, as well as neuroprotection in neurons of the nigrostriatal pathway of parkinsonian animals. We observed a reduction in GFAP+ levels in RES animals treated with CBD, as well as a reduction in α-syn levels. Classic markers of autophagy were decreased in parkinsonian animals treated with CBD. Cannabidiol was able to promote neuroprotection for motor and neurochemical symptoms through modulation of the autophagic pathway.