Aging is a complex biological process that often leads to cognitive decline, manifested as impairment of essential functions such as memory. Neurotransmission, essential for synaptic plasticity and cognitive function, is compromised in the aging brain, as well as in age-related neurodegenerative disorders. To characterize the relationship between age and cognitive function, a longitudinal study was conducted with C57BL/6 mice. Although this mouse strain has been the subject of multiple studies investigating various aspects of age-related cognitive decline, a comprehensive analysis of its associative and non-associative memory metrics across most of its lifespan is still lacking. Our behavioral studies describe physiological aging, examining mice from 3 to 26 months of age, using the open-field test (OFT) and the novel-object recognition (NOR) test in tandem. In the OFT, we observed age-dependent differences in the baseline movement of mice with a significant drop after 12-15 months of age. In addition, we detected significant changes in habituation to the context between 3 and 22-24 months of age. On the other hand, NOR results indicated significant changes between 6 and 18 months of age. Altogether, these results suggest that changes in associative and non-associative memories occur at different ages in mice. We managed to establish a baseline for the detection of memory impairments that could be used to evaluate the effectiveness of potential memory-potentiating agents.