Post-traumatic stress disorder (PTSD) arises after exposure to traumatic events and is associated with cognitive, emotional, and behavioral deficits. The single prolonged stress (SPS) model reproduces core PTSD symptoms in rats but has proven to not be as robust in mice. In this study we aimed to validate a murine version of SPS (mSPS) by characterizing the behavioral repertoire of mice through a battery of tests (Open Field, Light/Dark, Elevated Plus Maze, Y Maze, Tail Suspension). We searched for the most salient behaviors for which this model can be useful in mice, to allow it implementation in transgenic model available in our institution. Overall, mSPS did not produce robust changes in classical anxiety measures or global working memory but revealed sex and task specific effects. No significant anxiety differences were found in open field or plus maze, although females showed altered locomotion patterns and SPS mice displayed reduced peripheral exploration. In the light/dark test, stressed males entered the dark compartment faster, consistent with increased anxiety. In the tail suspension test, SPS mice, particularly females, showed increased and earlier onset of immobility. Together, these findings indicate that mSPS induces differential sex-specific alterations in exploratory dynamics and coping styles, highlighting the importance of sex-based analyses and broader behavioral assessments to support SPSm as a translational model for PTSD in mice.