The development and refinement of cortical circuits during the postnatal period depend on tightly coordinated transcriptional programs that regulate neural progenitor proliferation, neuronal differentiation, survival and maturation of postmitotic neurons. While the transcription factor Etv5 has been implicated in diverse neurodevelopmental processes, its specific contribution to postnatal cortical maturation remains poorly understood. In this study, we investigated the expression dynamics of Etv5 in the cerebral cortex during early postnatal development. In vivo analysis of cortical layers revealed no significant differences in the distribution of deep-layer (Ctip2⁺) or upper-layer (Satb2⁺) neurons between wild-type (WT) and conditional Etv5 knockout (cKO) mice, suggesting that Etv5 is not essential for initial layer specification. However, in primary cultures of mature cortical neurons, Etv5 expression increased progressively during in vitro maturation and was further upregulated in response to brain-derived neurotrophic factor (BDNF). These findings indicate that Etv5 may play a modulatory role in activity-dependent transcriptional programs during postnatal cortical development, rather than in early neuronal fate specification.