Perinatal life is known to be a high neuroplasticity period where social behavior and cognitive abilities are shaped. Moreover, several studies report neurochemical and behavioral benefits of the environmental enrichment (EE) in adults, but their impact on offspring gestated in these conditions remains underexplored. This study examines the influence of gestational EE on adolescent rats, focusing on their ability to counteract the adverse effects of postnatal stress and potential sex differences. For this, Wistar rats were exposed to EE or standard conditions during gestation. On postnatal days (PND) 1 to 21, pups were subjected to either maternal separation (SM) stress or no stress. At adolescence (PND 45-50), social behavior and spatial learning and memory were assessed using the three-chamber test and Barnes maze respectively. Blood corticosterone levels were assessed at the end of the experiment. EE positively affects social behavior by increasing the rate of visits and time spent with social stimuli. As regards spatial learning, almost all groups showed improvements in at least one indicator, suggesting that most learn to perform the task over time. However, when evaluating contextual memory, postnatal stress turned out to have a negative impact, which could not be overcome by EE. Finally, EE, on its own, increases corticosterone levels in both sexes. In sum, results showed that both treatments, independently, influence behavior and endocrine response in adolescent rats.