The dentate gyrus of the hippocampus generates adult-born granule cells (abGCs) throughout life, which integrate into pre-established neural circuits. Recently, we performed single-nuclei RNAseq for transcriptomic profiling of distinct abGCs cohorts. Combining differential gene expression, pseudotime trajectory and transcription factors (TFs) regulon analysis, we identified four cellular states: quiescent radial glia-like cells, proliferative progenitors, immature abGCs and mature abGCs. We propose that transitions between these states are driven by specific transcriptional regulators controlling the main features of each developmental stage. Thus, we manipulated the expression of key TFs as a strategy to unravel the molecular mechanisms underlying differentiation, maturation, integration and function of abGCs. Foxo1 is a TF predominantly expressed in quiescence and mature abGCs stages, but its function in establishing and maintaining the homeostasis of the mature neuronal phenotype is unknown. To address this question, we overexpressed Foxo1 in abGCs using retroviral constructs and analyzed neuronal morphology using confocal microscopy. Ten-day old cFoxo1-abGCs exhibited reduced dendritic length and branching points compared to control abGCs, which might lead to functional alterations. Other candidate genes highlighted by our regulon analysis are currently being manipulated to unveil their role in orchestrating neuronal state dynamics along abGC maturation.