Olfactory nerve injury induces cellular responses from myeloid immune cells and olfactory ensheathing cells (OECs), which may interact during the degenerative/regenerative process. Previous analyses revealed increased density and reduced morphological complexity of microglia/macrophages of the olfactory bulb, shortly after methimazole-induced injury. Here, we evaluated whether minocycline, a tetracycline antibiotic with known anti-inflammatory properties, modulates microglia/macrophage reactivity in response to olfactory nerve damage. Mice received oral minocycline (estimated in 50 mg/kg) or vehicle for seven days prior to methimazole administration, and microglia/macrophage responses were assessed in the olfactory bulb through immunohistochemistry against Iba1. Preliminary results suggest that minocycline per se did not have an effect on microglia/macrophage features in non-lesioned animals. In contrast, minocycline prevented the increase in microglia/macrophage density in the olfactory nerve layer, but did not prevent a reduction in cell complexity. These findings support the potential of minocycline as a modulator of a subset of microglia/macrophage responses in olfactory nerve injury at early stages after damage.