Neuromodulatory systems, such as dopaminergic, serotonergic and octopaminergic regulate motivation, learning, and memory in vertebrates and invertebrates. Preliminary studies indicate that cAMP-signaling facilitates context memory, presumably by neuromodulation. Our hypothesis is that neuromodulatory tone regulates generalization, understood as the application of learning to novel situations, but with some degree of similarity. To evaluate this, we trained Drosophila in one context and tested them in the same, in a similar, or in a different one, allowing us to distinguish between specific memory, normal generalization, and overgeneralization. Neural manipulation was performed using the GAL4/UAS system combined with cationic and anionic Channelrhodopsin targeting dopaminergic, serotonergic, octopaminergic neurons, motoneurons, and MBs. Inhibition of motoneurons validates the optogenetic strategy, as it causes paralysis and prevents context exploration. Preliminary results show that excitation of TH-dopaminergic neurons does not affect habituation memory, while their inhibition promotes it. In contrast, manipulation of PAM neurons (excitation or inhibition) has no effect. So far, no neuromodulator has affected recognition memory. However, inhibition of MB neurons does not alter habituation but does affect recognition memory. Overall, this strategy will allow us to test which neurons are required for learning generalization and possible overgeneralization.